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Combined Host Cell Proteins anticorps

Cet anticorps anti- est un anticorps Lapin Polyclonal détectant dans ELISA, WB et 2D-PAGE. Adapté pour E. coli.
Rockland
N° du produit ABIN6266436
N° du produit (Fournisseur): 200-401-m61

Aperçu rapide pour Combined Host Cell Proteins anticorps (ABIN6266436)

Antigène

Combined Host Cell Proteins

Reactivité

E. coli

Hôte

  • 1
Lapin

Clonalité

  • 1
Polyclonal

Conjugué

  • 1
Inconjugué

Application

ELISA, Western Blotting (WB), Two Dimensional Polyacrylamide Gel Electrophoresis (2D-PAGE)
  • N° du produit (Fournisseur)

    200-401-m61

    Fournisseur

    Rockland

    Fonction

    Anti-Host Cell Proteins Antibody recognizes high and low molecular weight protein fractions from E.coli.

    Attributs du produit

    Synonyms: rabbit anti-Host Cell Protein antibody, rabbit anti-HCP antibody, rabbit anti-E.Coli HCP antibody

    Purification

    Host Cell Protein A purified antibody is directed against high molecular weight and low molecular weight E.coli Host Cell Proteins.

    Stérilité

    Sterile filtered

    Immunogène

    Immunogen: Anti-Host Cell Proteins Antibody was prepared by repeated immunizations with a full range of high molecular weight (HMW) and low molecular weight (LMW) proteins from E.Coli (BL21).

    Immunogen Type: Native Protein

    Isotype

    IgG
  • Indications d'application

    Application Note: Anti-Host Cell Proteins Antibody detects over 300 E.coli proteins and has been tested by 2D-Page, western blotting, and ELISA.

    Western Blot Dilution: 1:500-1:1000

    ELISA Dilution: 1:1000-1:2000

    Restrictions

    For Research Use only
  • Format

    Liquid

    Concentration

    0.82 mg/mL

    Buffer

    Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2

    Stabilizer: None

    Preservative: 0.01 % (w/v) Sodium Azide

    Agent conservateur

    Sodium azide

    Précaution d'utilisation

    This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

    Stock

    4 °C,-20 °C

    Stockage commentaire

    Store vial at -20° C prior to opening. Aliquot contents and freeze at -20° C or below for extended storage. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use.

    Date de péremption

    12 months
  • Antigène

    Combined Host Cell Proteins

    Sujet

    Background: Anti-Host Cell Proteins Antibody recognizes high and low molecular weight protein fractions from E.coli. This antibody has been validated by 2D electrophoresis western blot to assure highest possible coverage of E.coli HCP. Host Cell Proteins are process-related impurities derived from a host cell expression system that may be present in trace amounts in a final drug substance. HCP Antibodies are used to detect impurities in biologically-based therapeutics. Detecting impurities often unseen by 1-D electrophoresis, a 2D electrophoresis western blot analysis delivers more complete insight into the immunocoverage of protein components of the host cell lysate.
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